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Cloning and Expression
Enzymes
MmeI
Supplier Images
MmeI
MmeI from New England Biolabs
(1)
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Citations:
(13)
Supplier Page
Supplier Page from
New England Biolabs for
MmeI
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Product Specs
Company Info
Citations
Reviews
Protocols
Product Specs
Item
MmeI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0637S
Concentration
2,000 U/ml
Quantity
100 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
TCCRAC(20/18)
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (13)
(1)
A dicentric bacterial chromosome requires XerC/D site-specific recombinases for resolution
Current Biology
August 1, 2022
Qin Liao, Zhongqing Ren, Emma E. Wiesler, Clay Fuqua, Xindan Wang
isolation using QIAGEN DNeasy blood & tissue kit (
69504
). 3 μg of gDNA was digested with
MmeI
(
NEB
R0637S
) for 90 min, and
quick CIP
(
NEB
M0525L
) for 60 min at 37°C. The DNA was extracted using Phenol-Chloroform,
(2)
Elucidation of the MicroRNA Transcriptome in Western Corn Rootworm Reveals Its Dynamic and Evolutionary Complexity
Genomics, Proteomics & Bioinformatics
October 1, 2021
Xiaozeng Yang, Elane Fishilevich, Marcelo A. German, Premchand Gandra, Robert E. McEwan, André Billion, Eileen Knorr, Andreas Vilcinskas, Kenneth E. Narva
cleaved 20 bp downstream of the 3′-adapter with Mme1 type II restriction endonuclease (Catalog No.
R0637S
,
New England Biolabs
, Ipswich, MA) and ligated onto a double-stranded 3′-DNA adapter. Ligated fragments
(3)
GRID-seq for comprehensive analysis of global RNA–chromatin interactions
Nature Protocols
July 1, 2019
Bing Zhou, Xiao Li, Daji Luo, Do-Hwan Lim, Yu Zhou, Xiang-Dong Fu
MEM non-essential amino acid solution (100
×;
Gibco
, cat. no.
11140076
)
MmeI
(
New England Biolabs
, cat. no.
R0637S
)
Dynabeads MyOne Streptavidin C1
(
Thermo Fisher Scientific
, cat. no.
65001
) New England Biolabs
(4)
High-salt–recovered sequences are associated with the active chromosomal compartment and with large ribonucleoprotein complexes including nuclear bodies
Genome Research
November 1, 2018
Marie-Odile Baudement et al.
volume of 325 µL) are added, as well as 76 µL of water and 4 µL of
MmeI
restriction enzyme (
NEB
, ref.
R0637S
; final volume of 100 µL). This reaction is incubated 90 min at 37°C under agitation. The supernatant
(5)
Decoding a Salmonella Typhi Regulatory Network that Controls Typhoid Toxin Expression within Human Cells
Cell Host & Microbe
January 10, 2018
Casey C. Fowler, Jorge E. Galán
10236276001
Terminal Transferase
,
New England BioLabs
,Cat #
M0315S
MmeI
,
New England BioLabs
,Cat #
R0637S
Sau3AI
,
New England BioLabs
,Cat #
R0169S
BamHI
,
New England BioLabs
,Cat #
R3136S
ONPG,Sigma-Aldrich,Cat
(6)
Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for Microorganisms
Current Protocols in Microbiology
February 2, 2015
Tim van Opijnen, David W. Lazinski, Andrew Camilli
1 mM Tris·Cl, pH 8.3 (APPENDIX 2A) 2 U/μl
MmeI
restriction enzyme (
New England Biolabs
, cat. no.
R0637S
) 32 mM S-adenosyl methionine (SAM; New England Biolabs) 10× Buffer 4 (New England Biolabs) Calf
(7)
Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for Microorganisms
Current Protocols in Molecular Biology
April 14, 2014
Tim van Opijnen, David W. Lazinski, Andrew Camilli
(Table 1) 1 mM Tris Cl, pH 8.3 (APPENDIX 2A)
MmeI
restriction enzyme (
New England Biolabs
, cat. no.
R0637S
) 32 mM S-adenosyl methionine (SAM; New England Biolabs)
CutSmart buffer
(
New England Biolabs
) Calf intestinal
(8)
miRNA regulation in the early development of barley seed
BMC Plant Biology
December 1, 2012
Julien Curaba, Andrew Spriggs, Jen Taylor, Zhongyi Li, Chris Helliwell
(5'-GTTCAGAGTTCTACAGTCCGAC-3') and P2 (5'-CGAGCACAGAATTAATACGACT-3'). Amplicons were digested by
MmeI
(
New England Biolabs
, #
R0637S
) and dephosphorylated by Shrimp Alkaline Phosphatase treatment (Roche #11758250001). Samples were run
(9)
Fitness analyses of all possible point mutations for regions of genes in yeast
Nature Protocols
July 1, 2012
Ryan Hietpas, Benjamin Roscoe, Li Jiang, Daniel N. A. Bolon
(10)
Identifying microbial fitness determinants by Insertion Sequencing (INSeq) using genome-wide transposon mutant libraries
Nature Protocols
December 1, 2011
Andrew L. Goodman, Meng Wu, Jeffrey I. Gordon
(3’ - 5’ exo-; 5 U/μL) (New England Biolabs, Cat. No.
M0212S
)
MmeI
(
New England Biolabs
, Cat. No.
R0637S
)
T4 DNA ligase
(2,000,000 U/mL) (
New England Biolabs
, Cat. No.
M0202T
) Agarose (Roche, Cat. No.
(11)
Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for Microorganisms
Current Protocols in Microbiology
November 6, 2010
Tim van Opijnen, Andrew Camilli
(Table 1E.3.1) 1 mM Tris·Cl, pH 8.3 (APPENDIX 2A)
MmeI
restriction enzyme (
New England Biolabs
, cat. no.
R0637S
) HHMI Author Manuscript 32 mM S-adenosyl methionine (SAM; New England Biolabs) Buffer 4 (New England
(12)
Chromatin Interaction Analysis Using Paired-End Tag Sequencing
Current Protocols in Molecular Biology
January 1, 2010
Melissa J. , Yuyuan , Chia-Lin , Xiaoan , Yijun
(13)
Polony DNA sequencing
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
November 1, 2006
Gregory J Porreca, Jay Shendure, George M Church
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Reviews
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(1)
4/3/2018
Diagnostic Restriction Mapping Of Plasmid Constructs
Triveni Mahendrappa
I have constructed plasmid for cloning purpose and it was 4010 bp in size. After transforming this plasmid into recipient E. coli cells, plasmid was prepped from few colonies and checked presence of ...
Read Review
<<
<
>
>>
Protocols
Chromatin Interaction Analysis Using Paired- End Tag Sequencing
January 01, 2010
Polony DNA Sequencing
October 01, 2006
MmeI from New England Biolabs
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